KMID : 0387720200310020119
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Korean Journal of Blood Transfusion 2020 Volume.31 No. 2 p.119 ~ p.130
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Improving Storage Policy in Korean Public Cord Blood Banks: Comparison of Quality between Long-Term and Short-Term Storage of Cord Blood
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Lee Mi-Nam
Kim Kyeong-Hee Kim Byoung-Gwon Goh Ri-Young Kim Jun-Nyun
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Abstract
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Background: The decreased use of cord blood units (CBU) due to improvements in haploidentical transplantation is a financial burden for public cord blood banks. Currently, there is no guidance regarding the length of cryopreservation of CBU in Korean public banks. The relative quality of long-term storage CB (LTCB) and short-term storage CB (STCB) needs to be evaluated to establish a storage policy.
Methods: Thirty-four and thirty-one units of CB cryopreserved for less than one year and up to 14¡15.5 years, respectively, in the Busan Gyeongnam Public Cord Blood Bank were assessed. The total nucleated cells (TNCs), CD34+ cell counts, and colony-forming units-granulocyte monocyte (CFU-GM) were examined. The cell viabilities were evaluated by Eosin-Y exclusion staining and 7-aminoactinomycin D flow cytometry. The number of stored Korean public CB units from 2000 to 2016 was determined and categorized according to TNCs.
Results: The post-thawing viability of the STCBs measured by flow cytometry was consistently higher than that of the LTCBs (TNCs, 62.5% vs 57.3%; MNCs, 93.1% vs 88.9%; CD34+ cells 95.7% vs 94.0%). The CD34+ cell viability was significantly higher in STCB (P=0.03). The CFU-GM after thawing was higher in STCBs (61.5¡¾23.4 vs 49.9¡¾22.8 [0.95 mm2] P=0.05). Of the 48,161 CB units stored until 2016, Dec, 9,493 (19.7%), which were stored until 2006, had been stored for more than 10 years.
Conclusion: LTCB with a low number of cells (<0.7¡¿109 cells) should be considered to exclude from storage for therapeutic purposes to improve the storage efficiency.
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KEYWORD
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Cord blood, Cryopreservation, CFU-GM, CD34, Viability, Public cord blood bank
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